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Mouse Jugular Vein Catheterization
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Imaging & Video Resources
Selected videos from the Hato Lab, highlighting teaching materials, intravital kidney imaging, and myeloid cell dynamics.
01 · Teaching Resources
Laboratory Techniques
These videos are provided as teaching resources.
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Manual Microdissection of Mouse Kidney Tubules
02 · Kidney Imaging
Intravital Kidney Imaging
Live imaging of kidney injury, glomerular structure, mitochondria, and renal tubules.
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Real-Time Acute Tubular Necrosis by Intravital Microscopy
Red: TMRM (mitochondrial membrane potential); Green: autofluorescence; Blue: DAPI (nuclei)
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Glomerulus
Red: Rhodamine-labeled LPS; Green: C57BL/6-Tg(CAG-EGFP)1Osb/J
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Mitochondria in the Kidney (1)
Red: photoactivated mitochondrial Dendra2; Green: mitochondrial Dendra2 (not photoactivated)
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Mitochondria in the Kidney (2)
Post-IRI. Bone marrow chimera generated by transplantation of mito-Dendra2 bone marrow into wild-type recipients. Green: mitochondrial Dendra2 (not photoactivated); dark green/orange: autofluorescence
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Renal Tubules (1)
Free calcein kinetics in the kidney. Systemically administered calcein is filtered by the glomeruli, generating a luminal bolus that passes from the S1 to S2 segments. Calcein is also secreted into the S2 segment. Myeloid cells are also labeled green due to prior administration of calcein-containing liposomes. Green: calcein
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Renal Tubules (2)
Green: FITC-labeled inulin administered intravenously. Related to PMID:21784899
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Renal Tubules (3)
Green: Alexa488-P22 (60-nm icosahedral virus-like protein cage). Related to PMID:31424427
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Intravital Fluorescence Lifetime Imaging
Green: pseudocolored tubular autofluorescence (originally acquired in the blue channel); Yellow, blue, purple, and pink: pseudocolored fluorescence lifetime regions identified by phasor analysis (right) and back-mapped onto the original blue-channel image (left). Related to PMID: 28250053
03 · Immune Cell Imaging
Myeloid Cells in the Kidney
Intravital imaging of myeloid cells and related fluorescent reporters in the kidney.
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Myeloid Cells in the Kidney (1)
Red: rhodamine-labeled Poly(I:C); Green: B6.129P-Cx3cr1tm1Litt/J (CX3CR1-EGFP); Blue: DAPI. Related to PMID: 25398784
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Myeloid Cells in the Kidney (2)
Red: rhodamine-labeled Poly(I:C); Green: B6.129P-Cx3cr1tm1Litt/J (CX3CR1-EGFP); Blue: DAPI
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Myeloid Cells in the Kidney (3)
Red: rhodamine-labeled Poly(I:C); Green: B6.129P-Cx3cr1tm1Litt/J (CX3CR1-EGFP); Blue: DAPI
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Myeloid Cells in the Kidney (4)
Red: rhodamine-labeled Poly(I:C); Green: B6.129P-Cx3cr1tm1Litt/J (CX3CR1-EGFP); Blue: DAPI
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Myeloid Cells in the Kidney (5)
Green: B6.129P-Cx3cr1tm1Litt/J (CX3CR1-EGFP)
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Myeloid Cells in the Kidney (6)
Red: rhodamine-labeled Poly(I:C); Green: B6.129P-Cx3cr1tm1Litt/J (CX3CR1-EGFP); Blue: DAPI. Individual cells are tracked using the indicated colors.